2019-07-31 09:06:042025-05-22 22:30:09
Biological materials
Mutant
MGNA-B159 (yjbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1158 NBRP B. subtilis, Japan]
GP2066 (''[[gene|sasA]] [[gene|sasB]] [[gene|relA]]::mls''), available in [SW|Jörg Stülke]'s lab
BKE11600 ([[gene|sasB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
BKK11600 ([[gene|sasB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
GP3467 ''sasB-6xHis-cat'', available in Jörg Stülke's lab
MGNA-B159 (yjbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1158 NBRP B. subtilis, Japan]
GP2066 (''[[gene|sasA]] [[gene|sasB]] [[gene|relA]]::mls''), available in [SW|Jörg Stülke]'s lab
BKE11600 ([[gene|sasB]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
BKK11600 ([[gene|sasB]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11600 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCATACATCCCCCAATTC, downstream forward: _UP4_CAATAGGTAAAGGGGAAGAA
The protein
Catalyzed reaction/ biological activity
ATP + GTP --> AMP + guanosine 3'-diphosphate 5'-triphosphate (according to UniProt)